thickness and prove the proliferating effects of these products on epidermal cells on the molecular level. Interestingly, HAderived products revealed a specific upregulation pattern of chemokines such as CXCL5 and CCL20, in contrast to PLLAbased injectable dermal fillers. These data suggest a secondary effect of the HA-based compounds on the immune cells of the skin, especially monocytes and macrophages. Moreover, we detected that primarily HA fillers with fine particles promote collagen synthesis, which in previous studies was associated with increased TGF-β signaling.3
So far, scientific knowledge about the combined use of HA fillers and laser treatment is still limited.4 Our results revealed synergistic effects of fractional ablative laser treatment and injection of HA dermal fillers supporting a combination of both treatments. This combined treatment promotes tissue modulators such as Serpin E1, which plays a key role in the cutaneous wound repair program.5
The present study showed that skin models are a reliable tool to investigate the molecular effects of dermal fillers and their combination with laser treatment.
So far, scientific knowledge about the combined use of HA fillers and laser treatment is still limited.4 Our results revealed synergistic effects of fractional ablative laser treatment and injection of HA dermal fillers supporting a combination of both treatments. This combined treatment promotes tissue modulators such as Serpin E1, which plays a key role in the cutaneous wound repair program.5
The present study showed that skin models are a reliable tool to investigate the molecular effects of dermal fillers and their combination with laser treatment.
DISCLOSURES
The authors have no relevant conflicts to report.
ACKNOWLEDGMENT
This work was partially supported by a grant from Galderma.
REFERENCES
1. Walker K, Basehore BM, Zito PM. Hyaluronic Acid. In: StatPearls. Treasure Island (FL)2019.
2. Marquardt Y, Amann PM, Heise R, et al. Characterization of a novel standardized human three-dimensional skin wound healing model using non-sequential fractional ultrapulsed CO2 laser treatments. Lasers Surg Med. 2015;47(3):257-265.
3. Fan Y, Choi TH, Chung JH, Jeon YK, Kim S. Hyaluronic acid-cross-linked filler stimulates collagen type 1 and elastic fiber synthesis in skin through the TGF-beta/Smad signaling pathway in a nude mouse model. J Plast Reconstr Aesthet Surg. 2019;72(8):1355-1362.
4. Urdiales-Galvez F, Martin-Sanchez S, Maiz-Jimenez M, Castellano-Miralla A, Lionetti-Leone L. Concomitant use of hyaluronic acid and laser in facial rejuvenation. Aesthetic Plast Surg. 2019;43(4):1061-1070.
5. Simone TM, Higgins CE, Czekay RP, et al. SERPINE1: A molecular switch in the proliferation-migration dichotomy in wound-"activated" keratinocytes. Adv Wound Care (New Rochelle). 2014;3(3):281-290.
2. Marquardt Y, Amann PM, Heise R, et al. Characterization of a novel standardized human three-dimensional skin wound healing model using non-sequential fractional ultrapulsed CO2 laser treatments. Lasers Surg Med. 2015;47(3):257-265.
3. Fan Y, Choi TH, Chung JH, Jeon YK, Kim S. Hyaluronic acid-cross-linked filler stimulates collagen type 1 and elastic fiber synthesis in skin through the TGF-beta/Smad signaling pathway in a nude mouse model. J Plast Reconstr Aesthet Surg. 2019;72(8):1355-1362.
4. Urdiales-Galvez F, Martin-Sanchez S, Maiz-Jimenez M, Castellano-Miralla A, Lionetti-Leone L. Concomitant use of hyaluronic acid and laser in facial rejuvenation. Aesthetic Plast Surg. 2019;43(4):1061-1070.
5. Simone TM, Higgins CE, Czekay RP, et al. SERPINE1: A molecular switch in the proliferation-migration dichotomy in wound-"activated" keratinocytes. Adv Wound Care (New Rochelle). 2014;3(3):281-290.
AUTHOR CORRESPONDENCE
Laura Huth PhD lhuth@ukaachen.de
