Clinical Evidence of Dermal and Epidermal Restructuring from a Biologically Active Growth Factor Serum for Skin Rejuvenation

March 2019 | Volume 18 | Issue 3 | Original Article | 290 | Copyright © 2019

Frank Barone MD FACS,a Sameer Bashey MD FAAD,b and Frederick W. Woodin Jr. BSc

aEvolv Plastic Surgery and Medical Aesthetics, Toledo, OH bZO Skin Centre, Beverly Hills, CA cZO Skin Health, Inc., Irvine, CA


Background: Topical skin care products use various technologies to promote skin repair. Growth factors of human, animal, and plant-derived origins have clinically demonstrated the ability to repair skin by promoting collagen, elastin, and glycosaminoglycan (GAG) production to reconstruct and reinforce skin’s extracellular matrix (ECM). Human skin cells respond to instructions from highly specialized proteins or hormones referred to as growth factors. These growth factors initiate cellular communication that instigates cellular replication, production, or proliferation. The production of elastin and collagen dermal connective fibers slows, and, with age, the regenerative rates of GAGs become delayed. These biological issues can be exacerbated by extrinsic factors such as sun exposure, pollutants, and various other factors. Growth factor-based products have become important topical treatment modalities for addressing signs of skin aging such as fine lines, deep wrinkles, dryness, laxity, and textural irregularities.

Objective: The aim of a 12-week clinical trial of a growth factor composition was to assess its effectiveness at restoring skin health through dermal and epidermal restructuring of aged skin.

Results: Data from expert grading, and from corneometer and cutometer evaluations, as well as 2D and 3D image analysis, reflected significant improvements in facial skin appearance, firmness, elasticity, and hydration. Elements that improved most dramatically in investigators’ assessments included radiance, firmness, tactile elasticity, textural smoothness, overall appearance, and crow’s feet. Ultrasound imaging showed continual increases in dermal and epidermal restructuring throughout the study duration. Subject assessments reflected positive product tolerability and positive perception across a broad range of efficacy attributes through 12 weeks of usage.

Conclusion: The results verified the ability of a multi-modal plant and enzymatically derived growth factor-based product to achieve skin rejuvenation improvements by stimulating dermal ECM and fibrous tissue regeneration to reduce fine lines and coarse wrinkles, and improve skin firmness and elasticity, while restoring skin to a properly hydrated state.

J Drugs Dermatol. 2019;18(3):290-295.

Purchase Original Article

Purchase a single fully formatted PDF of the original manuscript as it was published in the JDD.

Download the original manuscript as it was published in the JDD.

Contact a member of the JDD Sales Team to request a quote or purchase bulk reprints, e-prints or international translation requests.

To get access to JDD's full-text articles and archives, upgrade here.

Save an unformatted copy of this article for on-screen viewing.

Print the full-text of article as it appears on the JDD site.

→ proceed | ↑ close


As skin ages, problems emerge, including fine lines, deep wrinkles, sagging, dryness, and rough or uneven texture. It is further known that sun exposure, pollution, and lifestyle choices (such as smoking) exacerbate skin stress, causing collagen degradation, chronic inflammation, and other manifestations that can contribute to visual signs of aging. Meanwhile, the body’s intrinsic restorative mechanisms slow as we age, leading to faster collagen and elastin denaturation, increased inflammation, and loss of skin firmness and density.1Growth factors can regulate several important processes to treat and prevent the signs of skin aging. Key examples include cell replication, collagen production, and the reduction of inflammation.2,3 In clinical studies, applying growth factors topically has been shown to strengthen the skin’s ECM,  signs of photoaging by stimulating collagen and elastin, and trigger the skin’s innate ability to heal.3-7Advances in genomics and molecular biology have unveiled multiple pathways and methods of intervention in which aged and damaged skin health can be restored. Growth factors of multiple origins have been clinically proven to augment many of the biological pathways that naturally occur in the skin to restore aged and damaged skin. The use of plant-derived or enzymatically derived stem cells can offer preferred options, as they can be more sustainably cultivated versus human or animal-derived stem cells and are not inherently susceptible to the presence of disease-causing contaminants.Dermal cells are primarily responsible for the production of the structural framework within skin, which is primarily com

↑ back to top

Related Articles