Human Adipocyte Apoptosis Immediately Following High Frequency Focused Field Radio Frequency: Case Study
June 2015 | Volume 14 | Issue 6 | Case Reports | 622 | Copyright © June 2015
David McDaniel MDa and Paula Lozanova MDb
aMcDaniel Institute of Anti-Aging Research, Virginia Beach, VA
bDepartment of Dermatology and Venerology, Medical University, Sofia, Bulgaria
METHODS and MEASUREMENTS: Two healthy female subjects underwent abdominal skin and fat biopsies at baseline and after one treatment with a similar focused field high frequency RF device capable of 200 watts for 45 minutes. Biopsies were performed 1 hour post-treatment and were analyzed using the TUNEL method. Infrared imaging of the skin surface temperature was measured in both subjects. Thermocouple measurements at 1 and 2 cm were performed during the treatment cycle on a single subject.
RESULTS: Histologic apoptotic index (pre and 1 hour post) showed an average increase of 487% (6.5 to 31.7). Thermal imaging demonstrated an average surface temperature of 31.6C° pre-treatment and 39.2°C post-treatment. The 1 cm depth thermocouple showed an initial temperature of 40C° and reached a maximum of 45°C 15 minutes into the treatment. It remained stable at 45°C for the remaining 30 minutes treatment time. No adverse events were noted.
CONCLUSION: RF treatment induces an increase in apoptotic index in adipocytes 1 hour post-RF treatment. This is accompanied by a peak temperature of 45°C in the fat layer. Skin surface temperatures remain substantially lower than fat temperatures.
J Drugs Dermatol. 2015;14(6):622-623.