Comparison of the Effects of Contractubex® Gel in an Experimental Model of Scar Formation in Rats: An Immunohistochemical and Ultrastructural Study

January 2012 | Volume 11 | Issue 1 | Original Article | 74 | Copyright © January 2012


Mustafa T. Sahin MD,a Sevinc Inan MD,b Serap Ozturkcan MD,a Elif Guzel MDc Cemal Bilac MD,a Gülsen Giray MD,b Sevda Muftuoglu MDd

aDepartment of Dermatology, Celal Bayar University Medical Faculty, Manisa, Turkey bDepartment of Histology and Embryology, Celal Bayar University Medical Faculty, Manisa, Turkey cDepartment of Histology and Embryology, Istanbul University Cerrahpasa Medical Faculty, Istanbul, Turkey dDepartment of Histology and Embryology, Hacettepe University Medical Faculty, Ankara, Turkey

CONCLUSIONS

In our study, we compared and discussed immunohistochemical and ultrastructural effects of extractum cepae-heparin sodium-allantoin mixture, heparin sodium and allantoin in the rat model. Since the effects of the onion extract-based compound have not been deeply characterized so far, we could not find a study comparing immunohistochemical and ultrastructural effects of Contractubex gel, heparin sodium, and allantoin in any animal model in the literature. The reduced dermal immunoreactivity for laminin, fibronectin, and TGF-β was associated with heparin-, allantoin-, and Contractubex gel treatment in our study. The gel-based extractum cepae-heparin sodium-allantoin mixture was found to have apparent keratinolytic and collagenase effects. The thinnest epidermis of the four groups and normal-appearing epidermal cells that had completed keratinization showed that Contractubex gel had apparent keratinolytic effects. The following findings show the effect of Contractubex gel on collagenase: [1] dermal papillary formation occurred earlier in the second group comparing to the other groups; [2] connective tissue formed, consisting of loosely-structured collagen fibers and vessels in the papillary dermis (Fibrosis did not develop, and scar formation completed close to normal cutaneous morphology.), and [3] scar formation reduced more effectively than no treatment, heparin monotherapy, or allantoin monotherapy groups.
We can emphasize that Contractubex gel results in better reduction of scar formation, since TGF-β, laminin, and fibronectin immunoreactivities were mild or moderate. We conclude that wound healing with extractum cepae-heparin sodium-allantoin mixture is better than no treatment, heparin monotherapy, and allantoin monotherapy groups according to immunohistochemical and ultrastructural measurements.

DISCLOSURES

The authors have no relevant conflicts of interest to disclose.

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