Development of an Oregano-based Ointment With Anti-microbial Activity IncludingActivity Against Methicillin-resistant Staphlococcus aureus
April 2010 | Volume 9 | Issue 4 | Original Article | 377 | Copyright © April 2010
William Eng MD and Robert Norman DO
Increasing antibiotic resistance has prompted a search for new compounds with anti-microbial activity. In the authors’ previous study,
oregano extract was identified as one of the most potent anti-microbial compounds. The disk diffusion method was employed to
assess the degree of inhibition against various microorganisms, and the bacteriostatic or bactericidal mechanism of action. Disk diffusion
studies showed that oregano was found to be bacteriostatic for Staphylococcus aureus (S. aureus) and methicillin-resistant S.
aureus, (MRSA) but bacteriocidal for seven other microorganisms. Psuedomonas aeruginosa could not be inhibited by oregano. An
ointment consisting of 1–10% oregano could inhibit most organisms except for Proteus mirabilis and Proteus vulgaris, which required
20% and Pseudomonas which could not be inhibited even at the highest concentration of 80%. Oregano extracts can be formulated
into an ointment that shows broad antimicrobial activity. Additional testing to assess tissue toxicity and other adverse reactions would
be needed prior to human testing.